AT (Figure 7g; Extra file 4: Figure S4e) in a subpopulation of cells. The Ki67 proliferation index of in vivo grafted cells ranged from 1.5 to 5.3 , and no tumor formation or hyperproliferative activity was observed in the course of the whole in vivo period.Abbreviations 4OHT: 4hydroxy tamoxifen; ATRA: Alltrans retinoic acid; ChAT: Choline acetyltransferase; DAPT: N[N(3,5difluorophenacetyl)Lalanyl]Sphenylglycine tbutyl ester; eGFP: Enhanced green fluorescent protein; NL: Typical Locke buffer; SPC01: Spinal cord neural stem cell clone 01; VOCCs: Voltageoperated Ca2 channels. Competing interests JP can be a consultant to ReNeuron PLC. The other authors declare that they’ve no competing interests. Authors’ contributions GC generated the cell lines, carried out immunocytochemistry, PCR evaluation, karyotyping, and drafting of your manuscript. NR, TA, and PJ carried out grafting research in rat spinal cord and immunohistochemistry, and assisted together with the drafting on the manuscript. OF and GD carried out all calcium recordings and assisted with drafting with the manuscript. AJ assisted together with the acquisition and evaluation from the microarray data, and LP assisted with cell culture and immunocytochemistry. Each AJ and LP provided a vital appraisal of your manuscript. JP, ES, and ST conceived in the study, participated in its design and style and coordination, and helped to draft the manuscript. All authors study and approved the final manuscript. Acknowledgements Grants: AV0Z50390703, GA AV: IAA500390902, The Charles Wolfson Charitable trust, EU 6th Framework Programme. G. Dayanithi and O. Forostyak were supported by the grant P304/11/2373 in the Grant Agency with the Czech Republic. The antibodies Isl1, Lhx3, and En1 were developed by TM Jessell and S BrennerMorton. Pax6 was developed by A Kawakami, and Nkx6.1, created by OD Madsen, was obtained in the Developmental Research Hybridoma Bank developed below the auspices of your NICHD and maintained by The University of Iowa, Department of Biology, Iowa City, IA 52242, USA. We thank John Sindon and Erik Miljan for facilitating the generation of the SPC cell lines at ReNeuron plc, Dr. Michael Antoniou for delivering the UCOE lentiviral construct, and Dr. James Dutt, IEM, ASCR, for helpful discussions and crucial reading on the manuscript. Author facts 1 The James Black Centre, Division of Neuroscience, King’s College London, 125 Coldharbour Lane, London, UK. 2Institute of Experimental Medicine, ASCR, Prague, Czech Republic.Price of SulfoxFluor 3Department of Neuroscience, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic.2-Hydroxy-4-(hydroxymethyl)benzaldehyde Purity 4Institut National de la Santet de la Recherche M icale, Unitde recherche U710, Montpellier and Ecole Pratique des Hautes Etudes, UniversitMontpellier two, Paris F75007, France.PMID:23991096 Received: 17 December 2012 Revised: 28 April 2013 Accepted: 3 June 2013 Published: 7 JuneConclusions We generated immortalized neural stem cell lines from human fetal spinal cord; these retain the phenotypic traits of the tissue of origin even immediately after prolonged in vitro propagation and engraftment into lesioned rodent spinal cord. These cell lines hence represent a beneficial tool for studying V2 interneuron differentiation in vitro and for further examining the possible of human neural stem cells as cellular therapies for spinal cord injury.Cocks et al. Stem Cell Analysis Therapy 2013, four:69 http://stemcellres.com/content/4/3/Page 13 ofReferences 1. Conti L, Cattaneo E: Neural stem cell systems: physiological players or.
