Ot. LOD (3.3 * )/slope and LOQ (10 * )/slope had been calculated applying SD () of intercept and slope [Table 2].Repeatability and robustnessRepeatability of technique was checked by repetitive application of marker compound (n = six) and measurement of peak area at single concentration amount of 100 ng/spot, smaller mean RSD (0.091) value represents the excellent repeatability of strategy. The strategy was discovered to be robust when tested by slight transform in mobile-phase composition and saturation time.AcknowledgementThe authors are thankful to the Director, CSIR-NBRI, Lucknow, for supplying needed facilities during the course from the experiment. TheIdentification of elite chemotypeCluster evaluation (Past application, version 2.15) Hammer et al. 2001 of 5 samples depending on the content of colchicine and gloriosine [Figure 6]Figure 5: Quantitative estimation of colchicine and gloriosine in the samples of Gloriosa superba from eastern HimalayasFigure six: UPGMA dendrogram for collected population of Gloriosa superba based on the content of colchicine and gloriosineTable five: Recovery research of typical colchicine and gloriosine in samples of Gloriosa superba by high-performance thin-layer chromatography Amount of common Amount of normal present ( ) added ( ) Colchicine 0.097 25 0.097 50 0.097 100 Gloriosine 0.035 25 0.035 50 0.035 100 *Values in /10 ml dry weight basis. SD: Regular deviation Standard* Theoretical worth ( ) 25.097 50.097 100.097 25.035 50.035 one hundred.035 Observed worth ( ) 26.89 50.04 100.15 25.028 50.031 one hundred.01 Recovery ( ) 93.33 one hundred.11 99.94 100.02 100.00 one hundred.02 Mean recovery ( ) D 97.79.86 one hundred.023.SPharmacognosy Magazine, Volume 13, Challenge 51, July-September 2017 (Supplement 3)ANKITA MISRA, et al.: Simultaneous Quantification of Bioactive Alkaloids in G. superba authors are also thankful to the NASF, Indian Council of Agriculture Research, New Delhi, India, for financial help (No. 7938).12. Subbaratnam AV. Alkaloid constituents of Gloriosa superba L. J Sci Ind Res 1952;11 B:446-7 . 13. Subbaratnam AV. Studies on alkaloid constituents of Gloriosa superba Linn. J Sci Ind Res 1954;13:670-1. 14. Prajapati ND, Purohit SS, Sharma AK, Kumar T. A Handbook of Medicinal Plants.1217500-64-5 site Jodhpur: Agro Bios Publishers, India; 2003. p. 221-34. 15. Larsson S, R sted N. Reviewing Colchicaceae alkaloids Perspectives of evolution on medicinal chemistry. Curr Major Med Chem 2014;14:274-89. 16. Asolkar LV, Kakkar KK, Chakare OJ. Second Supplement to Glossary of Indian Medicinal Plant with Active Principles. Part-I (A-K). New Delhi: Publications and Facts Directorate, Council of Scientific and Industrial Investigation; 1992.Buy3-(Trimethylsilyl)-2-propyn-1-ol 17 Bharathi P Philomina D, Chakkaravarthi S.PMID:23671446 Estimation of colchicine in six various species of . , Gloriosa grown in vivo. Indian J Pharm Sci 2006;68:806-9. 18. Roberge CJ, Gaudry M, Gilbert C, Malawista SE, de M icis R, Lussier A, et al. Paradoxical effects of colchicine on the activation of human neutrophilis by chemotactic variables and inflammatory microcrystal. J Leukoc Biol 1996;59:864-71. 19. Srivastava S, Misra A, Shukla PK, Kumar B, Lata S, Rawat AK. A validated over pressured layered chromatography (OPLC) method for separation and quantification of colchicine in Gloriosa superba (L.) tubers from various geographical regions. RSC Adv 2014;4:60902-6. 20. Hadad GM, Badr JM, El-Nahriry K, Hassanean HA. Validated HPLC and HPTLC procedures for simultaneous determination of colchicine and khellin in pharmaceutical formulations. J Chrom.