Ed that the enhanced MF quantity observed in C57BL/6 mice as in comparison with BALB/c mice was reflective of an expanded F4/80hi population (Figure 1C). There were also much more F4/80lo MF inside the C57BL/6 strain but only at day 11 pi. F4/80lo MF numbers did not differ considerably among strains all through the remainder of your time course (Figure 1C). Notably, while C57BL/6 mice displayed elevated numbers of monocytes all through the infection as compared to their naive controls, BALB/c mice were marked by a important influx of Ly6C+ monocytes initial observed at day 35 pi (Figure 1C). Hence, the immune response for the parasite in C57BL/6 mice was characterised by enhanced numbers of F4/80hi MF and B cells from day 11 pi. In contrast, susceptible BALB/c mice showed significantly less F4/80hi cell expansion and an influx of Ly6C+ monocytes from day 35 pi.Campbell et al. eLife 2018;7:e30947. DOI: https://doi.org/10.7554/eLife.two ofResearch articleImmunologyFigure 1. Enhanced F4/80hi MF and B cell numbers are related with resistance. (A) Difference in total exudate cell, MF and B cell quantity amongst naive and L. sigmodontis infected C57BL/6 and BALB/c mice at day 11, 28, 35 and 50 pi. MF had been identified as live, Lin- (CD19, Ly6G, SiglecF, TCRb) CSF1R+ CD11c-.Formula of 1247542-90-0 (B) Representative plots from naive and infected BALB/c mice at d35 pi, demonstrating the gating technique utilised to determine and divide the MF population according to expression of F4/80, MHC and Ly6C. (C) Quantity of F4/80hi, F4/80lo and monocytes isolated from pleural cavity of mice in (A). Presented would be the data from two separate time course experiments (day 11 and 28 and day 35 and 50), every single of that is representative of 3 independent experiments with six mice/group/time point. *p0.05, **p0.01, ***p0.0001, ****p0.00001 as determined by a 2-way ANOVA comparing infected C57BL/6 with infected BALB/c mice at each time point. Error bars represent the mean SEM. DOI: https://doi.org/10.7554/eLife.30947.002 The following figure supplement is offered for figure 1: Figure supplement 1. Breakdown of cell populations inside the pleural exudate. DOI: https://doi.org/10.7554/eLife.30947.Campbell et al. eLife 2018;7:e30947. DOI: https://doi.org/10.7554/eLife.3 ofResearch articleImmunologySusceptible BALB/c mice fail to keep the F4/80hi resMF populationTo further characterise the MF dynamics inside the pleural cavity, we profiled the percentage contribution of F4/80hi, F4/80lo and monocyte populations for the MF compartment as a whole inside each strain of naive and infected animals (Figure 2A).4-Formyl-3-hydroxybenzoic acid site In naive C57BL/6 manage mice the F4/80hi population constituted 800 in the total MF population from 8 to 15 weeks of age (Figure 2B).PMID:32472497 The remaining 100 with the MF compartment was composed of F4/80lo MF, with almost negligible contribution of monocytes inside the C57BL/6 strain (Figure 2B). Interestingly, the proportion of F4/80hi cells contributing to the MF pool in naive C57BL/6 mice declined slightly with age (87 three to 73 three ) and infection prevented this age-related decline, keeping the F4/80hi population at 90 in the total MF pool (Figure 2B). The transcription element GATA6 and cell surface protein CD102 have been identified as markers of residency expressed by the F4/80hi MF population inside the peritoneal and pleural spaces of C57BL/6 mice (Okabe and Medzhitov, 2014; Rosas et al., 2014; Bain et al., 2016). Consistent with these reports the F4/80hi population in C57BL/6 mice was constructive for GATA6 and CD102 (Fi.
