Ifth exon.Supplemental DataThe following supplies are readily available inside the on-line version of this article. Supplemental Figure S1. A subset of subfamily 1 ARRs can functionally complement the arr1 arr12 mutant according to cytokinin-modulated hypocotyl elongation and seed size. Supplemental Figure S2. RT-PCR confirmation of transgene expression in transgenic lines of arr1 arr12. Supplemental Figure S3. T-DNA insertion mutants of subfamilies 2 and three have minimal effect on cytokinin response based on a root development dose response assay. Supplemental Figure S4. T-DNA insertion mutants of subfamilies 2 and 3 have minimal effect on cytokinin responses in hypocotyl elongation assays. Plant Physiol. Vol. 162,RNA Expression AnalysisTotal RNA was isolated and first-strand complementary DNA synthesis performed as previously described (Argyros et al., 2008). RT-PCR was applied toCharacterization of Type-B ARABIDOPSIS RESPONSE REGULATORSSupplemental Figure S5. Potential of subfamily 2 and 3 members of the family to functionally complement the arr1 arr12 mutant determined by cytokininmodulated hypocotyl elongation and seed size. Supplemental Table S1 Oligonucleotides applied for cloning type-B ARRs.9-Chloroacridine web Supplemental Table S2. Oligonucleotides used to examine expression of subfamily two and 3 T-DNA insertion lines. Supplemental Table S3. Oligonucleotides made use of for quantitative RT-PCR. Received October eight, 2012; accepted March 10, 2013; published March 12, 2013.LITERATURE CITEDAbe T, Futsuhara Y (1986) Genotype variability for callus formation and plant regeneration in rice (Oryza sativa L.). Theor Appl Genet 72: 3?0 Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, et al (2003) Genome-wide insertional mutagenesis of Arabidopsis thaliana.N-(3-Chloro-4-hydroxyphenyl)acetamide uses Science 301: 653?57 Argyros RD, Mathews DE, Chiang Y-H, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE (2008) Sort B response regulators of Arabidopsis play key roles in cytokinin signaling and plant improvement.PMID:23829314 Plant Cell 20: 2102?116 Bell CH, Porter SL, Strawson A, Stuart DI, Armitage JP (2010) Working with structural data to adjust the phosphotransfer specificity of a two-component chemotaxis signalling complicated. PLoS Biol 8: e1000306 Bent AF, Clough SJ (1998) Agrobacterium germ-line transformation: transformation of Arabidopsis without the need of tissue culture. In Gelvin SB, Schilperout RA, eds, Plant Molecular Biology Manual, Ed 2. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 1?4 Birch RG (1997) Plant transformation: troubles and approaches for sensible application. Annu Rev Plant Physiol Plant Mol Biol 48: 297?26 Birnbaum K, Shasha DE, Wang JY, Jung JW, Lambert GM, Galbraith DW, Benfey PN (2003) A gene expression map on the Arabidopsis root. Science 302: 1956?960 Candela M, Vel quez I, de la Cruz B, Sendino AM, de la Pe A (2001) Differences in in vitro plant regeneration capacity amongst four Arabidopsis thaliana ecotypes. In Vitro Cell Dev Biol Plant 37: 638?43 Dello Ioio R, Linhares FS, Sabatini S (2008a) Emerging role of cytokinin as a regulator of cellular differentiation. Curr Opin Plant Biol 11: 23?7 Dello Ioio R, Nakamura K, Moubayidin L, Perilli S, Taniguchi M, Morita MT, Aoyama T, Costantino P, Sabatini S (2008b) A genetic framework for the manage of cell division and differentiation in the root meristem. Science 322: 1380?384 Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard JF, Guindon S, Lefort V, Lescot M, et al (2008.